https://tryout.nlbif.nl/resource?r=pilotdunes pilotDunes Dr. Margarita Hernandez-Restrepo Westerdijk Fungal Biodiversity Institute

Utrecht 3584CT NL

m.hernandez@wi.knaw.nl Dr. Marcelo Sandoval-Denis Westerdijk Fungal Biodiversity Institute

Uppsalalaan 8 Utrecht 3584CT NL

m.sandoval@wi.knaw.nl Dr. Margarita Hernandez-Restrepo Westerdijk Fungal Biodiversity Institute

Uppsalalaan 8 Utrecht Utrecht 3584CT NL

m.hernandez@wi.knaw.nl http://wi.knaw.nl Dr. Marcelo Sandoval-Denis Westerdijk Fungal Biodiversity Institute

Utrecht Utrecht 3584CT NL

2025-11-12 eng Fungal isolations from soil in Dutch dunes as part of the pilot study from ARISE project Occurrence Fungi Soil Dunes Netherlands Cultures microfungi GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml Specimen LivingSpecimen GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction. Creative Commons Zero v1.0 Universal https://spdx.org/licenses/CC0-1.0.html CC0-1.0 https://tryout.nlbif.nl/archive.do?r=pilotdunes 3 plots were sampled (and divided in 96 squares) from each plot 3 soil samples were analysed. Location is approximate 4.364863 4.364863 52.16623 52.16623 2021-08-03 family Trichosphaeriaceae order Pleosporales family Pseudeurotiaceae class Eurotiomycetes family Clavicipitaceae family Myxotrichaceae family Chaetosphaeriaceae family Sympoventuriaceae family Tilletaciae family Cordycipitaceae family Pleosporaceae order Trichosphaeriales order Mycosphaerellales family Didymellaceae order Eurotiales family Bionectriaceae order Capnodiales class Exobasidiomycetes family Didymosphaeriaceae class Sordariomycetes phylum Ascomycota family Mycosphaerellaceae family Hypocreaceae order Xylariales family Ophiocordycipitaceae family Dissoconiaceae family Microdochiaceae family Herpotrichiellaceae kingdom Fungi order Hypocreales order Tilletiales family Niessliaceae family Teratosphaeriaceae family Diaporthaceae family Mortierellaceae phylum Mucoromycota order Chaetothyriales order Venturiales family Teichosporaceae family Neopyrenochaetaceae family Pyrenochaetopsidaceae order Microascales class Mortierellomycetes family Phaeosphaeriaceae order Thelebolales family Aspergillaceae family Lentitheciaceae family Microascaceae phylum Basidiomycota order Mortierellales family Cladosporiaceae order Helotiales family Stachybotryaceae order Chaetosphaeriales family Nectriaceae order Hyporeales class Dothideomycetes class Leotiomycetes order Diaporthales ARISE-BIODIVERSITY unknown Dr. Margarita Hernandez-Restrepo Westerdijk Fungal Biodiversity Institute

Uppsalalaan 8 Utrecht Utrecht 3584CT NL

m.hernandez@wi.knaw.nl http://wi.knaw.nl Dr. Marcelo Sandoval-Denis

NL

m.sandoval@wi.knaw.nl Soil samples were set to air-dry by opening the bags for 24 hours. 1 gr of soil was prepared in serial dilutions with sterile water, and different dilutions were plated in different agar media. After incubation at room temperature pure cultures were transfer to new media. Cultures were preserved at -20 C and DNA was extracted for molecular identification Dunes, single event For sampling simple corers of Ø2 cm and 20 cm deep made out of PVC tubes were used to take a total of 9 cores from the topsoil of approx. 50g, avoiding stones and organic materials when possible, and then collected into plastic Ziplock bags. Dr. Margarita Hernandez-Restrepo 0000-0001-5157-3392 originator Dr. Macelo Sandoval-Denis 0000-0001-6832-0252 originator 2025-11-12T14:38:39.061+00:00 dataset Hernandez-Restrepo M, Sandoval-Denis M (2025). fungal isolates from Dunes. Version 1.2. CBS Fungal Biodiversity Centre. Occurrence dataset. https://tryout.nlbif.nl/resource?r=pilotdunes&v=1.2 https://tryout.nlbif.nl/logo.do?r=pilotdunes https://tryout.nlbif.nl/resource?id=pilotdunes/v1.2.xml